It integrates the problems and solutions encountered in the HPLC application process, which is worth learning for novices! Example: 1. When doing HPLC analysis, the column pressure is unstable, what is the reason? How to solve it? 2. What is the main reason for the lack of sensitivity of HPLC and the solution? 3. Use and maintenance of liquid chromatography column 4. Analysis by HPLC The retention time sometimes drifts and sometimes changes rapidly. What is the reason? How to solve it? 5. I replaced the ODS column of another grade. Although the separation situation is still acceptable, the retention time cannot be reproduced.
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