If it is a water-proof incubator, formic acid fumigation can be used (formaldehyde reacts with potassium permanganate to form formic acid), and then the inner wall of the incubator can be wiped with 75% alcohol. The effect is better. If it is an air-jacketed incubator, wipe the inner wall of the incubator directly with 75% alcohol, or use water-proof fumigation, but the carbon dioxide and temperature probes must be closed before fumigation to prevent formic acid corrosion.
The CO2 incubator is a necessary instrument in the laboratory, but the high humidity due to the need to place a water container, which causes the rapid growth of mold spots (identified as filamentous fungi by culture) and affects the work, which is unsightly and may lead to experiments Sample contamination or spread of nosocomial infection. Although various disinfection measures such as copper sulfate solution, 75% ethanol solution or ultraviolet irradiation were used, there was still no significant effect. We found from the literature and practice that commercially available high-chloride disinfection tablets that are generally not suitable for use in this situation have the effect of safely and thoroughly removing such mildew spots when increasing the dose and using a neutralizer
Aseptic operation
(1) Before the experiment, the sterile room and the sterile operating table are irradiated with ultraviolet light for 30-60 minutes to sterilize, wipe the sterile operating table surface with 70% alcohol, and turn on the sterile operating table fan for 10 minutes before starting. Experimental operation. Only one cell is processed per operation to avoid cross-contamination of the cells. After the experiment, the experimental items are taken out of the workbench. If the next experiment needs to be continued, wipe the sterile operation table with 70% alcohol, and then Allow the aseptic operating table fan to run for 10 minutes before proceeding to the next experimental operation.
(2) The aseptic operation work area should be kept clean and spacious, and necessary items such as test tube racks, pipettes or pipette heads can be placed temporarily, and other experimental supplies should be removed in time to facilitate gas circulation. 70% alcohol can only be taken into the sterile operating table after wiping. The experimental operation should be carried out in the sterile area in the center of the operating table, and not in the non-sterile area on the edge.
(3) Take out the sterile experiment supplies carefully to avoid pollution. Do not touch the straw and the tip of the tip or the bottle mouth of the container, and do not operate the experiment directly above the opened container. After the container is opened, hold the cap and hold Hold the bottle, tilt it at an angle of about 45 °, and try not to place the bottle cap on the table top.
(4) Staff should pay attention to their own safety, and must wear experimental clothes and gloves before conducting the experiment. Special care should be taken for cell lines derived from human or virus infection, and select an appropriate level of sterile operating table (at least two levels) ). During the operation, avoid the generation of aerosols, be careful of toxic reagents, such as DMSO and TPA, and avoid sharp objects from hurting people.
(5) Regularly check the following items: CO2 pressure in the CO2 cylinder; whether the CO2 concentration, temperature, and water tray in the CO2 incubator are contaminated; whether the airflow pressure in the sterile operating table is normal, and replace the UV lamp and HEPA filter regularly Filter membrane, pre-filter (300 hours / pre-filter, 3000 hours / HEPA).
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