Quantitative Test Kit (ELISA) for Rat Total Carnitine (TC)
user's Guide
[Rat Total Carnitine (TC) Elisa Kit Kit Name]
Quantitative Test Kit (ELISA) for Rat Total Carnitine (TC)
[Use of rat total carnitine (TC) Elisa kit kit]
Quantitative detection of total carnitine (TC) in rat tissues, body fluids, urine and other related liquid samples.
ã€Test principle of rat total carnitine (TC) Elisa kit】
This kit uses double-antibody two-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the standard and the test sample to the pre-coated rat carnitine (TC) monoclonal antibody transparent enzyme label coated plate. After incubation for a sufficient time, wash to remove unbound components, and then add the enzyme label to work After incubation for a sufficient period of time, wash to remove unbound components. Substrates A and B were added in sequence, and the substrate (TMB) was converted into a blue product catalyzed by horseradish peroxidase (HRP), which turned yellow under the action of an acid. The concentration of carnitine (TC) is positively correlated. The OD value is measured at a wavelength of 450 nm. Based on the OD value of the standard and the sample, the total carnitine (TC) content in the sample is calculated.
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