The research group led by Professor Jiao Xin'an, Laboratory of Zoonoses and Immunology, School of Biological Science and Technology, Yangzhou University, has used modern molecular biology methods to develop an ELISA kit. The rapid detection of bacteria has been successful. Salmonella and Listeria monocytogenes are the two pathogens, and the entire detection process takes only two days, which is much faster than the national standard method, with high sensitivity and strong specificity, and the result analysis is simple.
ELISA kits are used for detection of different specific bands that appear after amplification of Listeria monocytogenes. The whole detection process takes about 24 hours, and samples can be taken anytime and anywhere to qualitatively detect Salmonella and Listeria monocytogenes in food.
The ELISA kit has the advantages of high specificity, sensitivity, fast and simple, and low cost. It can not only ensure the accuracy and reliability of sample detection, but also save a lot of manpower, material and financial resources. The social and economic benefits are extremely valuable for promotion and application. Can be widely used in food processing, market quarantine, entry and exit inspection and quarantine, hospital clinical testing and other industries and institutions. Biosensors provide a cheap and portable new tool for detecting biomolecules in fields such as genetic analysis, infectious disease detection, and food safety. Fan Chunhai's research group in the Department of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences has long been devoted to the research of electrochemical biosensors and has made a series of research progress. Recently, at the invitation of Accounts of Chemical Research, Pei Hao and Zuo Xiaolei wrote related review papers and published them in Acc. Chem. Res., 2014, 47, 550–559.
The assembly process of biomolecular probes on the sensing interface largely determines the performance of biosensor detection. How to control the density and orientation of biomolecules on the interface, reduce the non-specific adsorption of biomolecules and interfaces and avoid the lateral forces between the interface molecules have become one of the challenging problems in this field. In response to this problem, Fan Chunhai's group conducted a systematic study on the assembly process of DNA molecules at the macro and nano interfaces. Since DNA is a soft substance, the conventionally used one-dimensional single-stranded probes are prone to entangle between strands and aggregate. In 2003, a two-dimensional DNA probe with a certain rigidity was developed, which solved this problem to a certain extent (Fan et al. PNAS 2003, 100, 9134), and demonstrated in the detection of DNA and nucleic acid aptamers. Obvious advantages. In recent years, through the introduction of DNA nanotechnology, rigid three-dimensional structure DNA probes have been developed to achieve precise control of the distance between DNA probes. This provides a new way for constructing an orderly biomolecular interface, improves the biorecognition capability of the interface, and thus significantly improves the detection capability of the biosensor. On this new type of biosensing platform, the physicochemical mechanisms such as interface recognition and electron transport have been deeply studied, and ultra-high sensitivity detection of nucleic acids, antigens and small molecules has been achieved. The relevant results have been successively in JACS, Angew. Chem., Adv. Published by Mater., Sci. Rep. And other magazines.
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