Free ELISA test kit for human factor VIII

This reagent is for research use only: serum or plasma test principle: FVIII kit is a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA). Standards with known FVIII concentration and samples with unknown concentration are added to microwell enzyme plates To be tested. Incubate FVIII and biotin-labeled antibody first. After washing, HRP labeled with avidin was added. After incubation and washing, the unbound enzyme conjugate is removed, and then substrates A and B are added to act simultaneously with the enzyme conjugate. Produce colors. The color depth is proportional to the concentration of FVIII in the sample. Bring your own material distilled water. Sampler: 5ul, 10ul, 50ul, 100ul, 200ul, 500ul, 1000ul. Oscillator and magnetic stirrer etc. Safety Avoid direct contact with the stop solution and substrates A and B. Once exposed to these liquids, rinse with water as soon as possible. Do not eat, drink, smoke or use cosmetics during the experiment. Do not use your mouth to absorb any ingredients in the kit. Handling precautions Reagents should be stored according to label instructions, and returned to room temperature before use. The sparse standards should be discarded and cannot be stored. The slats not used in the experiment should be immediately returned to the packaging bag, sealed and stored to avoid deterioration. Unused other reagents should be packed or covered. Do not mix reagents of different batches. Use before warranty. Use a disposable pipette tip to avoid cross-contamination, and avoid using a sampler with a metal part when drawing the stop solution and substrates A and B. Use a clean plastic container to prepare the washing solution. Mix all components and samples in the kit thoroughly before use. When washing the enzyme-labeled plate, it should be fully patted dry. Substrate A should evaporate to avoid opening the lid for a long time. Substrate B is sensitive to light and avoid prolonged exposure to light. Avoid contact with hands, it is toxic. The OD value should be read immediately after the experiment is completed. The order of adding reagents should be the same to ensure that all wells are incubated for the same time. Perform the incubation operation according to the time, the amount and order of the liquid indicated in the instructions. Sample collection, processing and storage methods Serum-avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins. After collecting blood, centrifuge at 1000 × g for 10 minutes to separate the serum and red blood cells quickly and carefully. Plasma ----- EDTA, citrate, heparin plasma can be used for detection. Centrifuge at 1000 × g for 30 minutes to remove particles. The cell supernatant was centrifuged at 1000 × g for 10 minutes to remove particles and polymers. Tissue homogenate ----- Mash the tissue with appropriate amount of saline. Centrifuge at 1000 × g for 10 minutes, and take the supernatant for storage. If the sample is not used immediately, it should be divided into small parts and stored at -70 ℃ to avoid repeated freezing. If possible, do not use hemolysis or hyperlipidemia. If there are a large number of particles in the serum, centrifuge or filter before testing. Do not thaw at 37 ° C or higher. Thaw at room temperature and ensure that the sample is thawed evenly and adequately. Reagent preparation standards: Serial dilutions of standards should be prepared during the experiment and cannot be stored. Before dilution, the standard was mixed by shaking. Dilution of washing buffer (50 ×): 50-fold dilution with distilled water. Before use, mix all reagents thoroughly. Don't make the liquid generate a lot of foam, so as to avoid adding a large number of bubbles during sample addition, which will cause errors in sample addition. The number of slats required is determined by the number of samples to be tested plus the number of standard products. It is recommended to make multiple holes for each standard and blank hole. Each sample is determined according to its own quantity, and those that can use multiple holes can be used as much as possible. The specimen was diluted 1: 1 with the specimen dilution solution and then added 50ul to the reaction well. Add 50ul of the diluted standard to the reaction well and 50ul of the sample to be tested in the reaction well. Immediately add 50ul of biotin-labeled antibody. Cover the membrane plate, gently shake and mix, and incubate at 37 ° C for 1 hour. Shake off the liquid in the wells, fill each well with washing liquid, shake for 30 seconds, shake off the washing liquid, pat dry with absorbent paper. Repeat this operation 3 times. If washing with a plate washer, the number of washes is increased once. Add 80 ul of streptavidin-HRP to each well, gently shake and mix, and incubate at 37 ° C for 30 minutes. Shake off the liquid in the wells, fill each well with washing liquid, shake for 30 seconds, shake off the washing liquid, pat dry with absorbent paper. Repeat this operation 3 times. If washing with a plate washer, the number of washes is increased once. Add 50ul of substrate A and B to each well, mix gently by shaking, and incubate at 37 ° C for 10 minutes. Avoid light. Remove the enzyme labeling plate and quickly add 50ul of stop solution. After adding the stop solution, the results should be measured immediately. The OD value of each well was measured at a wavelength of 450 nm. The results above the No. 6 standard are non-linear, and accurate results cannot be obtained based on this standard curve. Kit performance 1. Sensitivity: The minimum detection concentration is less than No. 1 standard. Linearity of dilution. The correlation coefficient between the linear regression of the sample and the expected concentration is 0.990. 2. Specificity: Does not react with other cytokines. 3. Repeatability: The coefficients of variation within and between plates are less than 10%. Judgment and analysis of results 1. Instrument value: read the OD value of each well on a microplate reader with a wavelength of 450 nm. 2. Take the absorbance OD value as the ordinate (Y), and the corresponding FVIII standard concentration as the abscissa (X) To make the corresponding curve, the FVIII content of the sample can be converted to the corresponding concentration from the standard curve according to its OD value. 3. Detection range: 0-40ug / ml 4. Sensitivity: 0.1 ug / ml BC014-500ml DMEM / Ham's F-12 (1: 1) (DF-12) medium [Promotion] 500ml TC016-500ml L-15 medium L-15 medium 500mlBC018-500ml MEM 199 medium MEM 199 medium [promotion] 500mlBC019-500ml MEM 199 medium MEM 199 medium [promotion] 500mlBC020-500ml MEM 199 medium MEM 199 medium [promotion] 500mlBC021-500ml MEM 199 medium MEM 199 medium [Promotion] 500mlBC022-500ml MEM 199 medium MEM 199 medium [Promotion] 500mlBC023-500ml MEM 199 medium MEM 199 medium [Promotion] 500mlBC024-500ml MEM 199 medium MEM 199 medium [Promotion] 500ml CLS1056-200ml cell separation solution 1.056 200ml BC025-500ml RPMI 1640 medium RPMI 1640 medium [Promotion] 500ml BC026-500ml RPMI 1640 medium RPMI 1640 medium [Promotion] 500ml BC027-500ml RPMI 1640 medium RPMI 1640 medium [ Promotion】 500ml

Metal Zipper

Metal Zipper
Metal zipper zipper belongs to one of the microphones refers zipper teeth made of copper, aluminum or copper-nickel alloy material produced zipper, resin zipper and Nylon Zipper compared in terms of, more durable, and more for jeans, jackets and backpacks.

Features: Rugged, higher cost

Divided according to the size: # 3, # 4, # 5, # 7, # 8 and so on, depending on the size microphone size zipper teeth change. Because it is often used in the jeans, jackets and backpacks, so its dimensions are larger zipper.

classification:
1. TYPE: closed zipper,
Opening zipper (detachable),
Double closed end zipper (X- zipper with double slider zipper with two opposite or O- double slider two opposite), double open end zipper (Inverse open detachable)

2. Mi tooth Material: Existing material type of aluminum, brass, copper-nickel alloy, zinc alloy. Color metal microphone teeth often as needed electroplated, such as aluminum plated microphone yellow teeth, tooth-plated brass microphone white, black nickel, bronze, red bronze, gold and other colors.

Metal zipper Composition:
And other similar zippers, metal zippers composition is formed by a cloth belt, microphone teeth, slider, limit codes.
Tape: Material generally fiber belt, cotton or polyester fibers with a belt, can choose different materials depending on the application.
Mi teeth: the market for the production of metal zipper and more aluminum, brass, copper-nickel alloy, zinc alloy and other materials made of, since the color on the metal zipper teeth are plated microphone up, if improperly stored, the teeth will turn black microphone clothing and would cause pollution. For microphone tooth copper alloy is prone to oxidation, so when you save to ensure a certain degree of ventilation, do not sealed, do not save in the moisture-laden environment, if necessary, have to use the moisture-proof paper or desiccant.
Slider: metal slider respectively bronze color, red bronze plating, white, yellow-plated, black nickel, black nickel, matte silver, under normal circumstances, the color of the metal slider requires a microphone with a metal zipper teeth the same color. According to regulations, common slider parameters are:
3 # metal slider opening height of 2.1-2.2mm, mouth wide as 4.55-4.65
5 # metal slider opening height of 2.7-2.8mm, mouth wide 6.0-6.05
Limit code: divided into upper stop (front yard) and bottom dead center (post code). The main role is to prevent the upper and lower retaining slider from microphone teeth.

Note:
In the garment washing or processing time, pay attention to:
A wool knitwear after being ironed, to ensure adequate cooling can after zipper packaging, otherwise,
After the metal zipper will react with steam generating, easy to change color. Second, in the clothing trade processing, it tends to remain certain chemical reagents, then it is easy to react and metal zippers. Therefore, prior to assembly of metal zippers, leather has been the need to ensure adequate for cleaning and neutralization.
remind
Since there are different zip adaptability in different environments, so in the selection and purchase of the zipper when you choose must inform the manufacturer of the zipper on the application in which products, and manufacturers have to inform you of the fastener component requirements, such as You can be a pin detection and so on. Further, generally prohibit the use of azo dyes in the composition, as the dyed fastener after contact with body induce certain diseases.

Maintenance tips:
First, the science of using a metal zipper:
1, when the metal zipper pull, it must be close to both sides of the tooth, must be aligned to the top, then head down the track holds metal zipper pull gently, not too much force to pull.
Metal zipper
Metal zipper (2)
2, when moving the metal zipper should be careful not to hem skirts or other debris caught in the metal zipper in order to avoid "crooked teeth", "belly", "off the teeth" and so on.
3, if the metal zipper astringent, not easy to pull, do not dead lift, put some wax or soap on top of a metal zipper, you can pull.
4, if the metal zipper teeth unilateral differences rope, wear loose hair, available in popular tiger clamp closed end of the metal zipper lateral gently clip, metal zipper immediately changed for the better, unilateral teeth together tight, no broken loose.
Second, to prevent metal zipper moisture, oxidation, rust and discoloration.
1, to avoid contact with the moist environment: because the metal zipper metal oxide due to the dip caused by tooth portion has a black phenomenon.
2, avoid contact with rubber band: Because rubber band itself contains sulfides, when bundled with rubber band metal zipper, metal chain teeth will have vulcanized phenomenon (black).
3, should be cleaned and dried after washing with water: because the fabric dye or residual chemicals and metal parts redox reaction, metal parts may cause discoloration.

Cause of Color Changes
a, copper alloy (copper-nickel alloy, copper, brass) made of metal zipper discoloration of reasons:
When used in the metal or hair products, metal zippers metal due to oxidation caused most of the teeth have a black phenomenon. This is because when the bleach manufacturing process of cooked skin leather woolen products agents on the process and remains in the product, the product gas can cause discoloration of the metal zipper.
Examples of chemical reactions: oxidizing bleach (H2O2) → black (CuO) or red (CuO2);
b, rubber bands bundle of metal zipper color reasons:
Rubber band itself contains sulfides, when the rubber band bundle metal zipper, metal chain teeth will have vulcanized phenomenon (black).
Chemical Reaction Example: excess gas sulfides or HS2 conditions → black [CuS]
c, contact with an acid or chromium compound causes discoloration.

Chemical Reaction Example: acidic compound and chromium compound [Cr2O3] → Black [CuO, red [CuO2] or blue [CuSO4].

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