Preparations before use of planktonic sampler
Plankton samplers are widely used in the pharmaceutical industry, food industry, beverage tap water, drug inspection centers, health and epidemic prevention, hospitals, public places and other related industries and departments. It is a highly efficient porous suction sampler. It is based on the Anderson impact principle, and the impact velocity is 10.8m / s, which is equivalent to the fifth level of Anderson impact level. Today, we mainly introduce the preparatory work and sampling steps of the planktonic sampler before sampling.
1. Preparation before sampling of planktonic sampler
1. The temperature and humidity of the operating room (clean room) to be tested must meet the specified requirements; the static pressure difference, the number of air changes, and the air flow rate must be controlled within the specified values.
2. The unidirectional flow test should start no less than 10 minutes after the normal operation of the purification air-conditioning system; and no less than 30 minutes for the non-unidirectional flow.
3. During static test, there should be no more than 2 people in the room. The tester should be on the downwind side of the sampling port when sampling.
4. The tester should follow the aseptic procedure. Take all measures to prevent contamination of the sampling port and other artificial contamination of the sample.
5. The tester must wear clean clothes that meet the cleanliness level.
6. Before the plankton test, the operating room (clean room) under test has been sterilized.
2. Sampling steps of planktonic sampler
1. Prepare petri dishes (XX clean room, XX measuring point) in a clean room with a platform, and wipe the platform with medical absorbent cotton soaked in 75% ethanol before operation. The hands of the tester were strictly disinfected in the same way. During the preparation process, carefully check the quality of the culture medium plate again. If it is deteriorated, damaged or contaminated, it cannot be used.
2. Wipe the sampling port of the sampler, the sampling tube, the sample cover of the sampler, the inner and outer surfaces of the turntable and the cover with medical absorbent cotton soaked with 75% ethanol.
3. After the sampler is sterilized, do not put it in a Petri dish, and start the instrument to run in idling for not less than 5 minutes to evaporate the residual disinfectant in the instrument.
4. Set the sampling volume according to the cleanliness level of the operating room, the flow rate of the sampler (L / min) X sampling time (min) = sampling volume (L)
5. Place the Petri dish, cover the lid and adjust the height of the sampler gap. During the sampling operation, the petri dish should be handled lightly, and the outer diameter of the petri dish should be grasped with "five fingers", so as to avoid artificial contamination of the sample.
6. Arrangement of sampling points and number of sampling points.
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