Chromatography is a commonly used analytical method in modern biochemical experiments. Any chromatography process is carried out in two phases. One is the stationary phase fixed on the support, and the other is the mobile phase flowing through the stationary phase, due to the physical and chemical properties of the components in the sample (such as solubility, adsorption capacity, molecular shape, nature and quantity of charge carried by the molecule, molecules The specific groups, molecular weights, etc. of the surface are different, and the affinity for the stationary phase and the mobile phase is also different. When the hybrids pass through the porous support, they are also affected by the resistance of the stationary phase and the thrust of the mobile phase. The moving speed of each component is different and concentrated in different areas on the support, so that the components can be Separation.
Adsorption is the phenomenon in which certain substances are capable of concentrating solutes on their surface from solution. The adsorption capacity of the adsorbent is related to the chemical structure of the adsorbed material, the nature of the solvent and the nature of the adsorbent. When the solvent component around the adsorbent is changed, the affinity of the adsorbent to the adsorbed substance changes, and the adsorbed substance is released from the adsorbent. This release process is called "elution" or "spraying".
Adsorption chromatography involves loading the adsorbent into a glass column (column chromatography) or on a glass plate (thin layer chromatography). Since the adsorption capacity of the adsorbent can be changed by the influence of the solvent, the substance in the sample is adsorbed by the adsorbent, and then washed with an appropriate eluent to change the adsorption capacity of the adsorbent, desorb it, and move forward with the eluent. . When the desorbed material moves forward, it encounters the new adsorbent in front and is re-adsorbed. This adsorbed material is then released by the subsequent eluent. Through such repeated adsorption-desorption-resorption-re-desorption processes, the material can move along the advancing direction of the eluent. The speed of its movement depends on the adsorption capacity of the adsorbent on the material. Since the same adsorbent has different adsorption capacities for each component in the sample, the components are gradually separated due to different moving speeds during the elution process, which is the basic process of adsorption chromatography. Shanghai Chuangsai Technology provides 298-00-0|methyl parathion standard solution, Parathion-methyl, 500μg/ml, solvent: chloroform, commodity number: C16-P10773-1.2ml, price 155 yuan.
Solid adsorbents commonly used in experiments include alumina, magnesium silicate, calcium phosphate, calcium hydroxide, activated calcium, sucrose, cellulose, and starch. Commonly used eluents are ethane, phenethyl ether, chloroform, and various mixtures of ethanol, acetone or water with organic solvents. Adsorption chromatography is commonly used to separate non-polar and less polar organics such as lipids, steroids, carotenoids, chlorophyll, and their precursors. Shanghai Chuangsai Technology provides 67-64-1, acetone, Acetone, HPLC, commodity number: C28-A1213-4L, the price is 700 yuan.
It is worth mentioning that almost all solutes have a certain degree of adsorption for all chromatographic media, even inert materials. Except for the adsorption chromatography itself, the adsorption is more or less present in all other types. In the chromatography.
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